PSIONplusm is a webserver that predicts ion channels and their types, including voltage-gated channel for potassium, voltage-gated channel for sodium, voltage-gated channel for calcium, voltage-gated channel for anions, ligand-gated channel for potassium, ligand-gated channel for sodium, ligand-gated channel for calcium, ligand-gated channel for anion and non-ion-channel. The predictions are performed sequentially by combining the outputs generated with three SVM-based model from the PSIONplus method(PSIONION, PSIONVLG, PSIONVGS) and BLAST. This is the first method that performs multi-label ion channel type prediction, which means that one protein chain can be predicted as multiple ion channel types. For more details, please see our articles listed below.


Input your protein sequence in FASTA format.   Click for an example FASTA input
Or upload the sequence file:

  • Other information (optional)
  • > Select prediction type: (optional, the default is 5)
     1 (PSIONplus: discrimination for ion channel and non-ion channel)
     2 (PSIONplus: discrimination for voltage-gated and ligand-gated channel)
     3 (PSIONplus: discrimination for four types ion channel)
     4 (PSIONplusm: sequential prediction for single-label prediction )
     5 (PSIONplusm: sequential prediciton for multi-label prediction)


  • Gao J, Cui W, Sheng Y, Ruan J*, Kurgan L*.PSIONplus: Accurate Sequence-Based Predictor of Ion Channels and Their Types,PLoS One, 2016, 11(4):e0152964.
  • Gao J, Miao Z, Zhang Z, Wei H, Kurgan L. Prediction of Ion Channels and their Types from Protein Sequences: Comprehensive Review and Comparative Assessment. Current Drug Targets. 2019;20(5):579–592. doi:10.2174/1389450119666181022153942


    We thank these software DISOpred,PSIPRED,SPINE X,PSIBLAST for provide important features.